What is the standard dose of Streptozotocin used to induce diabetes in Rats?

Question

I been doing animal models for quite some time. Recently taken up Animal models in diabetic condition as the main topic. I had fixed several doses for inducing diabetes by STZ,50,55 and 60mg/kg.b.wt.As usual the animals were fasted overnight and STZ was prepared freshly along with normal saline. The chemical was than administered as ip .Blood glucose levels were raised up to 500mg/dl in 55 and 60mg/kg.b.wt but at 50mg/kg.b.wt the initial blood glucose levels were up to 150-200mg/dl,after the 7th day the blood glucose levels have come down to 65 and 70mg/dl, now at the 11th day it has risen to 1 - 2 folds. Is this the right dose.Iam confused.
I would request your good self to kindly help me in this regard and do the needful

Answer 1

Best Answer - Chosen by Voters

Just search Dr. Faustman, rats, and streptozotocin. You'll get hits like this: http://diabetes.diabetesjournals.org/cgi…

Answer 2

50mg/dl