Identify acetic acid, pyridinium hydrobromide perbromide and trans-cinnamic acid are a “reactant”, “product”,or“solvent” for this experiment?
1. Pre-heat your aluminum block to 70-80oC.
2. In a 5 mL conical vial add a “V-shaped” spin vane and 0.1 grams of trans-cinnamic acid. Add 0.5 mL of glacial acetic acid and warm to about 70-80oC to dissolve the cinnamic acid. Add 0.24 grams of pyridinium hydrobromide perbromide (PPB). Attach an air condenser to your flask and stir briskly at 70-80oC for 25 minutes. The reaction mixture will initially turn orange from the PPB, but will fade to an orange-yellow, clear solution during the reaction.
3. After 25 minutes, remove the vial from the hot plate, and place in a small beaker. Add 4 mL of ice-cold water to the reaction mixture, and stir with a spatula. Your product will precipitate as a thick, white or slightly yellow solid. Stir thoroughly for 1-2 minutes, then cool in ice for an additional 5 minutes.
4. Isolate the product by vacuum filtration on a Hirsch funnel. Rinse the reaction vial with 2-3 mL of ice-cold water to recover the remaining product and wash the filtered solid. Dry the solid in the funnel for 5 minutes.
5. Scrape your product onto a small watch glass, cover with a small piece of paper, and dry in an 80oC oven for 30 minutes. Determine the mass and melting range of the dry product. It is important that you determine the melting range slowly to get accurate data!!!1 AnswerChemistry3 days ago
Using this model, if there is one gene affecting skin color (gene A) with two alleles (A1A0), there’d be four possible genotypes: A1A1, A1A0, A0A1, and A0A0. According to the model, A1A0 and A0A1 would result in identical phenotypes, so there are three possible phenotypes: two pigment alleles (A1A1), one pigment allele (A1A0 and A0A1), and zero pigment alleles (A0A0).Biology2 weeks ago
But in your lab, you want to know how the gene effects human cell lines. You still aren’t sure if the disease is caused by the native (normal) gene not working correctly or by the mutated (disease version) working in a manner it shouldn’t.
You culture human kidney cells from native and mutated cells in tissue culture and want to remove the functioning gene from native cells.
What do you hope to learn by doing this?
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Looking for repeat structure not sure what that is ?1 AnswerChemistry2 months ago
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1. What is most likely the correct frame ?
2. What is the codon that specifies the first amino acid of the most likely reading from above ?2 AnswersBiology3 months ago
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Using NIH can’t figure out how !?1 AnswerBiology3 months ago