what is end capping in liquid chromatography?

what is end capping in liquid chromatography

3 Answers

Relevance
  • ?
    Lv 7
    9 years ago
    Favourite answer

    A column is said to be endcapped when a small silylating agent, such as trimethylchlorosilane, is used to bond residual silanol groups (-OH) on a packing surface. It is most often used with reversed-phase packings and may cut down on undesirable adsorption of basic or ionic compounds.

  • ?
    Lv 7
    9 years ago

    A reversed-phase HPLC column that is end-capped has gone through a secondary bonding step to cover unreacted silanols on the silica surface. End-capped packing materials eliminate unpredictable secondary interactions. Basic analytes tend to produce asymmetric tailed peaks on non end-capped columns, requiring the addition of modifiers to the mobile phase.

    http://www.discoverysciences.com/product.aspx?id=2...

  • 4 years ago

    END-CAPPED IS INCREASE LIFE OF COLUMN AND PROTECT THE SILIANE GROUP OF COLUMN AND PROTECTED TO DAMAGE OF COLUMN BY USING LOW & HIGH pH .

Still have questions? Get answers by asking now.