what is end capping in liquid chromatography?
what is end capping in liquid chromatography
- Favourite answer
A column is said to be endcapped when a small silylating agent, such as trimethylchlorosilane, is used to bond residual silanol groups (-OH) on a packing surface. It is most often used with reversed-phase packings and may cut down on undesirable adsorption of basic or ionic compounds.
A reversed-phase HPLC column that is end-capped has gone through a secondary bonding step to cover unreacted silanols on the silica surface. End-capped packing materials eliminate unpredictable secondary interactions. Basic analytes tend to produce asymmetric tailed peaks on non end-capped columns, requiring the addition of modifiers to the mobile phase.
- 4 years ago
END-CAPPED IS INCREASE LIFE OF COLUMN AND PROTECT THE SILIANE GROUP OF COLUMN AND PROTECTED TO DAMAGE OF COLUMN BY USING LOW & HIGH pH .